Chromosomal Aberrations and CYP1A1 Gene Study in Workers Exposed to Some Chemical pollutants in Erbil City
Summary
In this study the exposed individuals were selected in order to determine the effect of chemical pollutants on their Chromosomes and CYP1A1 Gene.
This Study included the targeted group of (72) individuals (48) from worker who exposed to some chemical pollutants like Benzene, Dust, Air pollutants ,(4) as a control group which have lung cancer and (20) control group.. In this study the exposed individuals were selected in order to determine the effect of chemical pollutants which exposed to workers during working
The cytogenetic study performed to (30) individual workers and (10 ) individuals as control and the G-banding was conducted for (8) individuals from both groups (workers and control) to indicate chromosome abnormalities which can be classed broadly into numerical (i.e., aneuploidy) and structural alterations (e.g., deletion, translocation, homogenously staining region (HSR), double minutes (DMs).
The molecular study was performed to all (72) individual which included (48)individuals from workers as exposed groups (4) as a positive control (exposed) which have lung cancer and 20 as a negative control group. Genetic polymorphisms of CYP1A1 were determined by the PCR-RFLP method. The molecular study examined whether polymorphisms of CYP1A1, which plays a role in the metabolic activation of polycyclic aromatic hydrocarbons (PAHs) and detection of heterozygous (T/C) and homozygous (C/C) mutations. After that, 10 samples were selected and chosen in each of the CYP1A1 heterozygous, homozygous and normal controls for conducting the sequence analysis technique to support the RFLP results.
Cytogenetic results indicated that (25) individual have different type of chromosome aberration. including (ring chromosome 17.04% , dicentric chromosome 9.64 %, quadrate 14.79 %, chromatid gap 13,18 %, chromatid break 16.07% , polyploidy 3.21% , centromere gap 15.43% and centromere break 13.82%).
GTG-banding results showed that was there’s no any deletion, insertion and substitution of studied samples (all normal 46XY).
Meta-analysis showed that the MspI and exon 7 polymorphisms of CYP1A1 m1 heterozygous and homozygous variants individually as well as synergistically showed significant association (P<0.05) with genetic damage.
The study has found a significant association of CYP1A1 m1 heterozygous (C/T)+homozygous (C/C) variants with genetic damage suggesting that these polymorphisms may modulate the effects of PAH exposure and chemical pollutants in occupational settings. Homozygous wild type (T/T ) = affected 62%, positive controls 75%, negative controls 95% . heterozygous mutated type (C/T ) = affected 25,%, positive controls 0%, negative controls 5%. Homozygous mutated type (C/C)= affected 12.5,%, positive controls 25%, negative controls 0% , and sequence result indicated that homozygous and heterozygous mutations were present .
Posted:21/05/2017