Cytogenetic and Molecular Study of BRCA2 Gene Exon 21 of the Gastric Cancer Patients in Erbil City-Kurdistan Region -Iraq

 

SUMMARY

This study has been performed on (40) individuals which included (25) gastric cancer patients with (15) controls within a specific period, during September 2015 to April 2016 in both Nanakali and Rizgari Hospital in Erbil city. The Cytogenetic and Molecular study were performed for patients were suffering from gastric cancer and controls.

In the present study, the following results were obtained of gastric cancer patients
and controls:
1- Chromosomal aberrations: visible changes to chromosome structure and morphology have played a very important part as indicators of genetic damage in both clinical and cancer studies. In current study Chromosomal aberrations were observed in most patients who suffering from gastric cancer in both males and females at different age groups. which including (Dicentric chromosome %14.555, chromosomal break with fragment %11.96, chromatid gap %10.181 , chromatid break % 8.230 , chromosomal gap %7.806 , and ring chromosome %6.748) and no mutation detected for controls.
2-Molecular study: This study also included mutational analysis for BRCA2 gene –exon-21 as a novel study for indicating gastric cancer gene. DNA extracted for (40) individuals from both gastric cancer patients and controls in Erbil city, to check the prevalence of certain founder mutations in BRCA2 gene exon 21.Analysis of DNA purity and quantity was performed by using a Nano dropspectrophotometer and the PCR amplification was performed on the genomic DNA with pairs of primers spanning the exon region of the BRCA2 gene. By direct sequence analysis in the gene BRCA2 of exon 21 in our study for (10) patients sample of our sequence of PCR template for our target region, but no mutation detected in sequence of PCR template for our target region after comparing with reference sequence .

3- In our study several factors were studied as founder effectors of the gastric cancer such as, H.pylori infection, smoking, age, and sex, which had a role in developing gastric cancer and their effect were significance (P _ 0.05) except blood group had no effect.

 Posted : 23/ Jan/2017